Obtained by subtracting the initial OD600_oh (starting time = 0 h) in the measured OD600_x h at particular time points. the initial OD600_oh (beginning time = 0 h) from the measured OD600_x h at particular time points.(b) (b)(c) (c)3.4. Cytocompatibility Test 3.4. Cytocompatibility Test three.four.1. Cytotoxicity of Ga(NO3) three.2HGaHAp, only the highest Ga-containing powder (8 GaHAp) Inside the case of 1 mg/mL 2O three.four.1. Cytotoxicity of Ga(NO3) three.2H2aeruginosa growth (Figure)6a). With remedy is(Figure 6b) and showed inhibitory effects on in the The cell viability of hTERT-BJ1P. O presence of Ga(NO3 three.2H2O 2 mg/mL shown 4 cell fast reduce GaHAp, stronger inhibitory effect was observed, is shown The eight. Aviability of 6c) in cell viability was observed soon after the initial option and when in Figuremg/mL (FigurehTERT-BJ1 inathe presence of Ga(NO3)3.2H2Oday, beginning from applying four 8. Ga(NO reduce (p viability of observed immediately after the first day, O above the in Figuremg/mL (Figure 6c), in cell0.001). Concentrations of development 3with four GaHAp, from 150 g/mL A rapid3)3.2H2O total inhibitionwasP. aeruginosa Ga(NO )three.2H2starting six.three GaHAp, and Ga(NO3) .2H2O (p 0.001). Concentrations detected three)3.2H2 3 days, 150 g/mL 8 GaHAp3was observed. Development inhibition wasof Ga(NOwith the O above the MIC (75 g/mL) exhibited an increased toxicity already on the initially day. Aftersame concentration of 4 GaHAp and 6 GaHAp against S. three.2H(Figure 7c), S.G-CSF Protein Source epidermidis (Figure S3), S.CXCL16 Protein Species pyogenes MIC (75 g/mL) exhibited g/mL Ga(NO3)aureus 2O showed maintained metabolic days, only the cells exposed to 75an increased toxicity already on the first day.PMID:24578169 Following threeactiv(Figure S4), andto 75 g/mL Ga(NO3)three.2H2O showed maintained metabolic activonly the cells exposed E. coli (Figure S5). ity, around 80 . ity, about 80 . 3.four. Cytocompatibility Test 3.four.1. Cytotoxicity of Ga(NO3 ) three .2H2 OThe cell viability of hTERT-BJ1 inside the presence of Ga(NO3 )3 .2H2 O answer is shown in Figure eight. A fast decrease in cell viability was observed after the very first day, starting from 150 /mL Ga(NO3 )three .2H2 O (p 0.001). Concentrations of Ga(NO3 )3 .2H2 O abovethe initial OD600_oh (starting time = 0 h) from the measured OD600_x h at distinct time points.3.four. Cytocompatibility Test 3.four.1. Cytotoxicity of Ga(NO3) 3.2H2OJ. Funct. Biomater. 2023, 14, 51 10 of 15 The cell viability of hTERT-BJ1 in the presence of Ga(NO3)three.2H2O answer is shown in Figure eight. A rapid decrease in cell viability was observed following the very first day, beginning from 150 g/mL Ga(NO3)3.2H2O (p 0.001). Concentrations of Ga(NO3)three.2H2O above the MIC (75 (75 /mL) exhibited an elevated toxicity currently on theday. Following Just after three the MIC g/mL) exhibited an elevated toxicity currently on the first 1st day. three days, only only the cells exposed g/mL Ga(NO3)3.2H2.2H2 O showed maintained metabolic days, the cells exposed to 75 to 75 /mL Ga(NO3 )three O showed maintained metabolic activity, about 80 . activity, around 80 .J. Funct. Biomater. 2023, 13, x FOR PEER REVIEW11 ofFigure 8. Metabolic activity of human fibroblasts (hTERT-BJ1) exposed to various concentrations Figure 8. Metabolic activity of human fibroblasts (hTERT-BJ1) exposed to unique concentrations of Ga(NO )three .2H O solutions, where PC–positive handle. Damaging control final results were omitted of Ga(NO33)three.2H22 Osolutions, where PC–positive control. Adverse control final results have been omitted from the graphs as the the values have been around 0 .(One-way ANOVA single factor with Tukey’s fro.