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All living cells method information by trafficking cargo, for example extracellular ligands, microorganisms, nutrients, transmembrane proteins and lipids from the plasma membrane to endocytic vesicles (i.e. endocytosis). A reciprocal procedure called recycling balances endocytosis and returns significantly from the internalized membrane and cargo to the cell surface. The balance in between endocytosis and recycling controls the plasma membrane composition and gives cells with info that has been resolved in time and space. Endocytosis and recycling are master regulators of diverse cellular functions which include nutrient uptake and metabolism, improvement, proliferation, differentiation and polarity, 1-3 reprogramming, migration, cell adhesion and migration, cytokinesis, and neurotransmission . Endocytic and recycling pathways are very dynamic and very coordinated and let cells to turn over the equivalent with the entire plasma membrane 1-5x per hour. The cell-based L-glutahione protection assays are valuable to study endocytosis and recycling of transmembrane proteins like receptors, 4-8 channels, transporters, and adhesion molecules in epithelial and nonepithelial cells . We’ve previously studied endocytosis and recycling 9-15 from the LIMK2 Inhibitor list Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in human airway epithelial cells and HEK293 cells . The biotinylationbased assays described in the manuscript are optimized for examining endocytosis and recycling in epithelial cells cultured under polarizing circumstances on semipermeable growth supports. These protocols is often modified to study endocytosis and recycling of proteins in epithelial cells cultured in plastic tissue culture dishes or in nonepithelial cells. Figures 1 and 2 include examples of endocytic and recycling assays in epithelial and nonepithelial cells.