S transhepatic drainage was the only alternative. Not too long ago,endosonography (EUS) uided drainage was recommended as an alternative. Nevertheless,the approach is still in evolution Aims Techniques Aim: To assess the efficacy and security of unique methods in EUSguided biliary drainage. Individuals and Techniques: We collected the information of individuals treated more than months. The information integrated baseline characteristics,indications,strategies,success rates and complications. Sufferers were classified in accordance with the various sorts of approaches shown in Table. Within the first months,we employed tapered ERCP catheter,and in case it fails,needleknife sphincterotomy for inital tract dilation and plastic or metal stents sooner or later. For the final 5 months,we revised our strategy so that we utilised exclusively F cystotome. We preferred CDS more than HGS in distal obstructions in longscope position.A P THE POSSIBILITY OF LIQUID BIOPSY WITH BILE JUICE IN Sufferers WITH GALLBLADDER CANCER H. Kinugasa,,K. Matsumoto,K. Tsutsumi,H. Kato,K. Nouso,,H. Okada Gastroenterology and hepatology,Okayama University Graduate College of Medicine,Dentistry,and Pharmaceutical Sciences,Okayama,Gastroenterology and hepatology,Hiroshima City Hiroshima Citizens Hospital,Hiroshima,Gastroenterology,Okayama Citizens Hospital,Okayama,Japan Get in touch with Email Address: gyacygmail Introduction: Gallbladder cancer (GBCa) is amongst the most tough to treat cancers within the field of gastroenterology. To attain superior treatment impact in future,so referred to as “custommade” therapy primarily based around the character with the every single tumor is mandatory. Nevertheless,GBCa tissue cannot be obtained very easily. In this study,we attempt to elucidate the possibility of “liquid biopsy” with bile juice on the idea of non invasive diagnostic system as circulating tumor DNA in blood. Aims Procedures: Eleven individuals with GBCa enrolled in this study. Surgically removed tumors and their surrounding regular tissue were analyzed for mutations of oncogenes (Cancer panel; Haloplex,Agilent Technologies) by subsequent generation sequencing (NGS; Illumina,San Diego,CA,USA) in all individuals. Bile juice obtained from of patients before the therapy was examined simultaneously. As adverse controls,more healthful gallbladder tissue samples have been analyzed. Outcomes: We set cutoff value at for uncommon mutation price. TP mutation was detected inof the tumor tissue. Mutations of CTNNB,MET,AR were detected in ( ( ( samples,respectively. In these mutationpositive samples,greater than of the DNA was mutated in . of TP (EK). of TP (RK) and . of CTNNB (TI). None with the corresponding normal SR9011 (hydrochloride) tissues and controls had any mutations. Interestingly,we could detect the identical TP mutation even in bile juice as liquid biopsy in patients who had TP mutation in tumor tissue (;. Conclusion: Tumor DNA in bile juice could possibly be detected by NGS. It might enable us to produce a brand new genetic diagnosis of GBCa. Disclosure of Interest: None declaredUnited European Gastroenterology Journal (S) in BS group and in US group (ns). The early pancreatobiliary complication rate was in BS group and in US group (ns). The imply survival time was days PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/19389808 in BS group and days in US group (ns). The imply SF time was days in BS group and days in US group (ns,p.). The biliary reintervention was successfully performed on both groups. Other late complications were noted in US group only. Conclusion: Judging from these information,it is actually suggested that the bilateral MS deployment is the procedure to attempt very first for the patients with HBS because of.