The smallest protease has been confirmed to be a single experienced enzyme of E495 by N-terminal sequence examination and its molecular weight (MW) has been determined by MS to be 33.4 kDa in our previous study [three]
The uncooked CD knowledge had been transformed into suggest residue ellipticity (deg cm2 dmol21) at the total wavelength using the relation: l = hlM0/10/c.Substrate binding of GST-fused PPC domains was…