Product Name :
UbcH8 (human), (recombinant) (His-tag)
Sequence:
Purity:
≥95% (SDS-PAGE)
Molecular Weight:
~18 kDa
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Use/Stability :
Description:
UbcH8 is a class I ubiquitin-conjugating (E2) enzyme highly similar in structure to UbcH7. UbcH8 has been demonstrated to act in conjunction with Parkin, a ring-finger-containing E3 ubiquitin protein ligase implicated in the pathogenesis of autosomal recessive familial Parkinson’s Disease, and with Staring, an ring-finger E3 ligase responsible for ubiquitinylation of syntaxin 1. UbcH8 is a primary target gene of interferon-α and γ, and recent studies have indicated that UbcH8 is the E2 enzyme for the ubiquitin-like protein ISG15 (also known as UCRP).154447-36-6 Formula These studies suggest overlap between the ISG15 and ubiquitin conjugation pathways, and have important implications for understanding the regulation and function of ISG15 in the IFN α/β response.500285-30-3 MedChemExpress Western Blot analysis of BML-UW9135: Lane 1: MW Marker, Lane 2: 100ng of UbcH8 (human), (recombinant) (His-tag) probed with Anti-6X His-tag® antibody.PMID:30137793 SDS-PAGE analysis of Prod. No. BML-UW9135: Lane 1: MW marker, Lane 2: 2µg UbcH8 (human), (recombinant) (His-tag), Lane 3: MW marker. Western Blot analysis of BML-UW9135: Lane 1: MW Marker, Lane 2: 100ng of UbcH8 (human), (recombinant) (His-tag) probed with Anti-6X His-tag® antibody. SDS-PAGE analysis of Prod. No. BML-UW9135: Lane 1: MW marker, Lane 2: 2µg UbcH8 (human), (recombinant) (His-tag), Lane 3: MW marker.
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Solubility:
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Additional Information :
| Biological Activity This enzyme supports ubiquitinylation in vitro. | Formulation Liquid. In 20mM TRIS-HCl, pH 8.0, containing 0.5mM DTT. | MW ~18 kDa | Purity ≥95% (SDS-PAGE) | Source Produced in E. coli. | Technical Info / Product Notes Typical enzyme concentration to support conjugation in vitro is 100nM to 1µM depending upon conditions. | UniProt ID O14933