Name :
Recombinant Mouse CD39L3/ENTPD3 Protein (His Tag)
Biological Activity :
Background :
Biological Activity :
Testing in progress
Expression Host :
Mouse
Source :
Baculovirus-Insect Cells
Tag :
Protein Accession No. :
NP_848791.3
NCBI Gene ID :
Synonyms :
Synonyms :
ectonucleoside triphosphate diphosphohydrolase 3
Amino Acid Sequence :
Molecular Weight :
The recombinant mouse ENTPD3 consists of 453 amino acids and predicts a molecular mass of 50.9 kDa. It migrates as an approximately 55.8 kDa band in SDS-PAGE under reducing conditions.
Purity :
> 90 % as determined by SDS-PAGE.
State of Matter :
Product Concentration :
Storage and Stability :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Endotoxin Level :
< 1.0 EU per μg protein as determined by the LAL method.
Protein Construction :
A DNA sequence encoding the mouse ENTPD3 (NP_848791.3) (Gln44-Pro485) was expressed with a polyhistidine tag at the C-terminus.
Buffer Solution :
Lyophilized from sterile 20 mM Tris, 300 mM NaCl, pH 7.5, 10 % glycerol.Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Redissolution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Synonyms :
CD39l3 Protein, Mouse; HB6 Protein, Mouse; NTPDase-3 Protein, Mouse CD39L3/ENTPD3 背景信息
References & Citations :
Fausther M, et al. (2010) Cystic fibrosis remodels the regulation of purinergic signaling by NTPDase1 (CD39) and NTPDase3. Am J Physiol Lung Cell Mol Physiol. 298(6): 804-18.Vlajkovic SM, et al. (2006) Noise-induced up-regulation of NTPDase3 expression in the rat cochlea: Implications for auditory transmission and cochlear protection. Brain Res. 1104(1): 55-63.Ivanenkov w, et al. (2005) Characterization of disulfide bonds in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): implications for NTPDase structural modeling. Biochemistry. 44 (25): 8998-9012.
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