Monocyte Chemoattractant Protein-1 (MCP-1/CCL2) 8016

Additional Information:
accession P13500|express systemE.coli|product tagnone|purity> 97% by SDS PAGE|molecular weightPredicted Molecular Mass: 8.666 kDa Extinction Coefficient: 8,730 M-1 cm-1 Actual Molecular Mass (Mass Spec): 8.666 kDa by ESI Mass Spec|available size10 g, 100 g, 1mg, 2 g, 50 g|endotoxinsequenceQPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSIDHLDKQTQTPKT|Monocyte Chemoattractant Protein-1 (MCP-1/CCL2) 8016proteinDatabase link:human P13500Size and concentration5, 20, 50, 100, 1000g and lyophilizedFormLyophilizedStorage InstructionsAvoid repeated freeze-thaw cycles: 12 months from date of receipt, -20 to -70 C as supplied. 1 month, 2 to 8 C under sterile conditions after reconstitution. 3 months, -20 to -70 C under sterile conditions after reconstitutionStorage bufferReconstitution: Spin sample prior to reconstitution. Recommended concentration of 100g/mL in sterile water.Shipping: Room TempPurity> 97% by SDS PAGE and HPLCtarget relevanceMonocyte chemoattractant protein-1 (MCP-1) (CCL2) is produced by many cell types at sites of inflammation. It regulates chemotaxis and transendothielial migration of monocytes, as well as memory T cells and natural killer cells by interacting with their membrane surface receptor CCR2. MCP-1 has also been implicated in a number of disease states, such as rheumatoid arthritis, psoriasis, atherosclerosis, autoimmune diseases, tumor progression, and HIV infection. This cytokine displays activity for monocytes and basophils but not for neutrophils or eosinophils. It binds to chemokine receptors CCR2 and CCR4.Protein namesC-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2)Gene namesCCL2,CCL2 MCP1 SCYA2Protein familyIntercrine beta (chemokine CC) familyMass9606DaFunctionActs as a ligand for C-C chemokine receptor CCR2 (PubMed:10529171, PubMed:10587439, PubMed:9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed:10587439, PubMed:9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed:8195247, PubMed:8627182, PubMed:9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed:8107690).Subellular locationSecreted .TissuesExpressed in the seminal plasma, endometrial fluid and follicular fluid (at protein level) (PubMed:23765988). Expressed in monocytes (PubMed:2513477).StructureMonomer or homodimer; in equilibrium (PubMed:15033992, PubMed:8898111, PubMed:8989326, PubMed:9837883). Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans (PubMed:9792674). Interacts with TNFAIP6 (via Link domain).Post-translational modificationProcessing at the N-terminus can regulate receptor and target cell selectivity (PubMed:8627182). Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant (PubMed:8627182).; N-Glycosylated.Target Relevance information above includes information from UniProt accession: P13500The UniProt Consortium|