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Nscriptome to discover biological functions. Conclusions: Here, we present the first systematic analysis of ABC transporters in S. miltiorrhiza and predict candidate transporters involved in bioactive compound transportation in this important medicinal plant. Making use of genome-wide identification, transcriptome profile evaluation, and phylogenetic relationships, this research provides a brand new point of view on the essential functions of ABC transporters in S. miltiorrhiza. Keyword phrases: Salvia miltiorrhiza, Transporters, ATP-binding cassette (ABC) transporters, Gene family members evaluation, Tanshinone and salvianolic acid transport Correspondence: [email protected] Engineering Study Center of Chinese Medicine Resource, Ministry of Education, Institute of Medicinal Plant αLβ2 Antagonist list Improvement, Chinese Academy of Medical Sciences Peking Union Healthcare College, Beijing, ChinaThe Author(s). 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, so long as you give acceptable credit towards the mTORC1 Inhibitor web original author(s) along with the supply, present a hyperlink to the Creative Commons licence, and indicate if adjustments have been created. The pictures or other third celebration material within this short article are integrated within the article’s Inventive Commons licence, unless indicated otherwise in a credit line for the material. If material just isn’t integrated within the article’s Creative Commons licence as well as your intended use isn’t permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission straight from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies towards the data produced readily available in this short article, unless otherwise stated inside a credit line towards the data.Yan et al. BMC Genomics(2021) 22:Web page two ofBackground Salvia miltiorrhiza is actually a widespread medicinal plant made use of to treat inflammation and cardiovascular illnesses due to the fact of its high quantities of biologically active hydrophilic salvianolic acid (SA) and lipophilic diterpenoids (tanshinones) in its roots or rhizomes [1]. S. miltiorrhiza is an best model medicinal plant for studying secondary metabolic biosynthesis. GGPP is definitely the biosynthetic precursor of tanshinone, which is catalysed by copalyl diphosphate synthase (CPS) to type copalyl diphosphate. Then a series of cytochrome P450 monooxygenases (CYP450s) catalyses downstream oxidation reactions. Ferruginol, the catalytic product of CYP76AH1, is an essential intermediate product inside the biosynthetic pathway of tanshinone [2]. CYP76AH3 and CYP76AK1 are accountable for the conversion of ferruginol into intermediate compounds 11,20-dihydroxy ferruginol and 11,20-dihydroxy sugiol en route to becoming tanshinones [3]. SA biosynthesis is derived from 4-coumaroyl3,4-dihydroxyphenyllactic acid (4C-DHPL), that is a combination of three,4-dihydroxyphenyllactic acid (DHPL) and 4-coumaroyl-CoA. These two compounds are coupled by rosmarinic acid synthase (SmRAS) [4]. The 3-hydroxyl group is introduced by a cytochrome P450dependent monooxygenase (SmCYP98A14) to type rosmarinic acid [4]. Significant progress has been produced inside the understanding of the biosynthetic pathways of these active ingredients in S. miltiorrhiza, but the transport and storage mechanisms of those compounds in plant cells have not yet been elucida.